To study the influence of hydrogenation on the hot deformation behavior of Ti65 high-temperature titanium alloy, the high temperature compression tests of the samples with different hydrogen contents in the α+β two-phase region and β single-phase region were carried out by hot simulation experiment machine Gleeble-3500, respectively. The results show that the hydrogenated and unhydrogenated samples have similar rheological characteristics in the two-phase region and single-phase region, and the main softening mechanism in the two-phase region is dynamic recrystallization (DRX) of α phase and in the single-phase region is dynamic recovery (DRV) of β phase. Hydrogenation promotes the dynamic recrystallization softening of Ti65 high-temperature titanium alloy, and the deformation resistance of Ti65 high-temperature titanium alloy at 910 ℃ and 1 s-1 reduces near 104 MPa by adding 0.13 wt.% hydrogen, which significantly improves the hot workability of Ti65 high-temperature titanium alloy. Based on the experimental results, the Arrhenius constitutive equation is constructed by polynomial fitting method, and the relationships between various parameters and hydrogen content are determined. The constitutive equations of Ti65 high-temperature titanium alloy coupled with hydrogen content in the α+β two-phase region and β single-phase region are established by the polynomial fitting, and the average relative absolute errors of these equations are 9.41% and 6.68%, respectively. Thus, the established constitutive equations can accurately predict the rheological stress of hydrogenated Ti65 high-temperature titanium alloy, and provide a theoretical basis for formulating and optimizing the forming process of locally hydrogenated titanium alloy parts.