The deformation characteristics during the manufacturing process of W-shaped metal sealing rings were simulated by applying tensile deformations of 5%, 15% and 25%, and annealing heat treatment after deformation to nickel-based superalloy GH4169 rolled plate. Then, the uniaxial tensile tests under the strain rates of 0.1, 0.01 and 0.001 s-1 were conducted on the deformed and heat-treated nickel-based superalloy GH4169, and the influence laws of tensile deformation and heat treatment on the mechanical behavior, such as strain rate sensitivity and fatigue response of nickel-based superalloy GH4169 were investigated by combining microstructural characterization and fatigue testing. The results show that in terms of tensile deformation, the material exhibits significant hardening during the tensile deformation process, and the larger the deformation, the higher the yield strength after unloading and then stretching, the smaller the hardening rate, the stronger the strain rate sensitivity. However, the tensile deformation has little effect on the elastic modulus and ultimate strength of the material, and the hardening rate is independent of the strain rate. Regarding heat treatment, annealing at 950 ℃ increases the average grain size and texture intensity of the 15% tensile-deformed specimens, and accompanied by the formation of a small number of annealing twins. After annealing, the yield strength of specimens reduces, and the plasticity and hardening rate increase, but the strain rate sensitivity decreases, with the mechanical properties between the undeformed and tensile-deformed states. In terms of fatigue response, the annealing heat treatment reduces the accumulation of plastic strain under the action of fatigue loading, thereby improves the fatigue performance of the material, which appears a more significant improvement effect in the early stages of fatigue cycle.