The Febased cladding layer without defect was prepared on the surface of 45 steel plate by the different process parameters, and the microstructures of the cladding layer crosssection，fusion face and heat affected zone were examined by metallographic microscope. Furthermore, the hardness of the cladding layer crosssection was measured by Vickers hardness tester, the hardness curve was plotted, and the bonding strength between cladding layer and 45 steel plate was tested by universal testing machine. The results show that the microstructure of the cladding layer is mainly columnar grain with fine and dense microstructure without pores, cracks and slag inclusions in the inner cladding layer, and the hardness of the cladding layer is distributed regionally, namely, the hardness near the surface layer is higher than 730 HV0.3 and the hardness near the fusion face is higher than 600 HV0.3. The bonding strength between the cladding layer and the substrate is higher than that of the forged 45 steel, and the thickness of the heat affected zone is about 600-700 μm. Finally, the industrial application of laser cladding strengthening on the surface of automobile die is carried out, which shows the feasibility and advancement of laser cladding strengthening on automobile die.